Generator

Part:BBa_K779601

Designed by: Giulio Alighieri   Group: iGEM12_MIT   (2012-09-29)

Hef1a-mKate MammoBlock Device

A composite part with a Hef1A constitutive promoter with a mammalian-codon optimized red fluorescent protein. This protein is a significantly engineered reporter protein that, unlikely many fluorescent proteins, is monomeric and therefore ideal for fusion constructs.

MKatechar.png
200,000 cells were transiently transfected with 500 ng of Hef1A:mKate using Lipofectamine 2000. Images were taken on an Evos microscope at 10X. The left image is brightfield. The right is in the Texas-Red channel. Very high transfection efficiency and mKate expression levels can be observed when mKate is driven by the constitutive promoter Hef1A (BBa_K779200).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 569
    Illegal XhoI site found at 968
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal NgoMIV site found at 703
    Illegal AgeI site found at 81
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1837
    Illegal SapI.rc site found at 1219


[edit]
Categories
Parameters
n/aHef1a-mKate MammoBlock Device